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KMID : 0903520070500040268
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2007 Volume.50 No. 4 p.268 ~ p.275
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Microbial Population Diversity of the Mud Flat in Suncheon Bay Based on 16S rDNA Sequences and Extracellular Enzyme Activities
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Kim Yu-Jeong
Kim Sung-Kyum
Kwon Eun-Ju
Baik Keun-Sik
Kim Jung-Ho
Kim Hoon
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Abstract
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Diversity of the mud flat microbial population in Suncheon Bay was investigated by studying extra- cellular enzyme activities and 16S rDNA sequences. Four culturable bacterial strains with CMCase, xylanase and protease activities were isolated from the wetland and the mud flat. All the strains produced more xylanase activity than CMCase or protease activity, and the properties of the isolate enzymes from the wetland were similar to those from the mud flat. About 2,000 clones were obtained with the 16S rDNA amplified from the metagenomic DNA isolated from the mud sam- ples. Based on the restriction pattern(s), seventeen clones were selected for base sequence analysis. Of the 17 clones, only 35% (6 clones) were found to be cultured strains and 65% (11 clones) to be uncultured strains. The similarities in the base sequences of the clones ranged from 91.0% to 99.9% with an average similarity of 97.3%. The clones could be divided into 7 groups, Proteobac- teria (9 clones, 52.9%), Firmicutes (3 clones, 17.6%), Bacteroidetes (1 clone), Flavobacteria (1 clone), Verrucomicrobia (1 clone), Acidobacteria (1 clone), and Chloroflexi (1 clone). Most of the Proteobac- teria clones were gamma Proteobacteria associated with oxidation-reduction of sulfur.
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KEYWORD
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mud flat metagenome, 16S rDNA, microbial diversity, Proteobacteria, unculturable microorganisms
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